Our People

Brian David Bower, PhD 

(Scientific Consultant / Contractor)

SUMMARY: Dr. Bower has been helping meet the scientific and technical consulting and contracting needs of clients in and around the Raleigh-Durham-Chapel Hill and Research Triangle Park (RTP) area of North Carolina since 2017. He has a further 10 years of related experience from work at the University of Michigan (U-M), the University of North Carolina at Chapel Hill (UNC-CH), at Ohio University (OU) in the Edison Biotechnology Institute (EBI). His academic and commercial career has included a track record of success working in both small and large laboratories, with diverse multinational teams and in completely independent settings. Some of these success stem from his excellent verbal and written communication skills, including funded grant applications, peer reviewed publications, and award winning PowerPoint and poster presentations presented at domestic and international conferences.

Find Dr. Bower on LinkedIn

Dr. Bower's Personal LinkedIn Page can be found at:

Marketable and relevant experience and expertise include:


  • Molecular cloning techniques including cloning scheme development for protein and gene expression and targeting vectors
  • Backbone sourcing and modification
  • Adapter primer design and polymerase chain reaction (PCR) condition optimization
  • Restriction digestion
  • Dephosphorylation via Calf Intestinal Phosphatase (CIP) and Shrimp Alkaline Phosphatase (SAP)
  • Ligation via T4 Ligase
  • Related bacteriology skills (below)


  • Routine laboratory bacteriology to include production of competent cells
  • Plasmid transformation via heat shock and electroporation
  •  Plating and streaking for isolation
  • Colony picking and blue-white selection
  • Expansion in liquid culture including optical density monitoring
  • Plasmid DNA purification via methods including mini-, midi-, and maxi-preps Cesium Chloride (CsCl) gradient banding, and crude purification via alkaline lysis.
  • Procedures also include those relevant to protein expression and purification (below)


  • Transgenic protein expression induction using Isopropyl β- d-1-thiogalactopyranoside (IPTG)
  • Bacterial cell lysis using lysozyme, benzonase, DNAse, RNAse, sonication, and other mechanical means
  • Lysate separation via ultracentrifugation.
  • Protein purification via conventional and fast protein liquid chromatography (FLPC, generally using GE ÄKTA)
  • Protein quantification via Bradford assays
  • Analysis of protein purity via Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) followed by Coomassie Brilliant Blue staining
  • Buffer exchange via dialysis


  • Routine and specialized cell culture techniques including isolation of primary skin fibroblasts
  • Handling of a wide variety of primary and immortalized cells
  • Splitting / subculturing adherent cells via typsinization
  • Cell counting using both conventional hymocytometers and automated systems (e.g. the Countess II)
  • Seeding / inoculation of plates and flasks
  • Preparation and revival of cryopreserved cells.


  • Animal husbandry responsibilities to include matings, weanings, and euthansia
  • Collection of blood, serum and plasma from live mice
  • Blood gluclose monitoring in live mice, to include during insulin / IGF-1 tolerance tests
  • Analysis of live-animal body composition via benchtop NMR (e.g. via Bruker MiniSpec)
  • Development and performance of novel PCR based genotyping procedures for genetically modified mouse lines
  • Dissection of a wide variety of mouse tissues
  • Pulverization / homogenization of tissues for subsequent RNA and protein analyses


  • Conceive of, develop, and execute cell-culture experiments to examine drug kinetics, dynamics, interactions, serum-shift properties, and impacts upon biological pathways of interest via protein and RNA analyses (below).
  • Execution of concurrent of subsequent analyses in animal models as appropriate.


  • Preparation of lysates / protein extracts from cultured cells and pulverized/homogenized tissues
  • Literature and market review of available and proprietary antibodies
  • Production of dephosphorylated negative controls via treatment with lambda protein phosphatase (LPP/ λPP)
  • Optimization of extraction ratios, protein loading, and primary and secondary antibody dilution levels during development of florescent (e.g. via LI-COR Odyssey CLx) and chemiluminescent western blots (e.g. via Fisher iBright FL1000), and capillary electrophoresis based immunoblots (e.g. via Protein Simple Wes)
  • Quantification with ImageJ, ImageStudio, and other software, and subsequent data analysis.


  • Purification of RNA from cultured cells and pulverized/homogenized tissues using Trizol and Qiagen RNeasy kits
  • Quantification of RNA via NanoDrop or QuantIt RiboGreen assays
  • Analysis of RNA integrity via Agilent 2100 bioanalyzer
  • Generation of complimentary DNA (cDNA) via several commercial reverse transcriptase (RT) kits
  • Generation of appropriate negative controls
  • Analysis of assay limit of detection, linearity and amplification efficiency
  • Measurement of cDNA abundance via SYBR Green and TaqMan / probe-based method (e.g. via an Applied Biosystems StepOnePlus) and subsequent data analysis


  • Experience working in accordance with Environmental Protection Agency (EPA) Good Laboratory Procedures (GLPs) and the Federal Insecticide Fungicide and Rodenticide Act (FIFRA)
  • Experience working in accordance with Food and Drug Administration (FDA) GLPs including an ISO13485 compliant quality management system (QMS)
  • Followed, reviewed, authored and amended Standard Operating Procedures (SOPs)
  • Experience working in a Biosafety Level 2 (BSL2) laboratory
  • Familiar with Health Insurance Privacy and Portability Act (HIPAA) requirements from work in university health systems
  • Performed work in support of deregulation Cargill's Latitude Crop Product (produced in collaboration with BASF corp): https://www.cargill.com/page/latitude


  • Isolation of unknown but pathogenic human viruses from patient clinical samples
  • Amplification of viruses through cell culture to include examination of cytopathic effect (CPE)
  • Serotyping and titration of viruses in fixed cells via immunostaining and light microscopy
  • General procedures for safely working with such viruses in a Biosafety Level 2 (BSL2) laboratory


Dr. Bower continues to develop a variety of skills for the benefits of both his clients and BDB LLC.
While these skills may not be at an expert level, they may serve as useful adjuncts for a client’s project.

  • Software Development / Programming: Java, Python, SAS, C#
  • Web Development: HTML, CSS, JavaScript, PHP, web server configuration
  • Database Programming / Management: SQL, MySQL, Microsoft Access
  • Real Estate: Property record research, property acquisition, holding and resale
  • Finance: Creation of Expert Advisor (EA) systems on a hobby basis

Trademark and Copyright Notices and Disclaimers:

"BDB LLC" and the stylized "BDB" logo are trademarks™ of Brian David Bower LLC (doing business as BDB LLC). All other product and company names are trademarks™ or registered® trademarks of their respective holders, and they are used here under the doctrines of descriptive fair use pursuant to 15 U.S.C. § 1115(b)(4) and nominative fair use pursuant to 15 U.S.C. § 1125(c)(3)(A), and caselaw related thereto. No claim is made to the exclusive right to use such trademarks apart from the marks as shown. Use of them does not imply any affiliation with or endorsement by them. These trademark holders are not affiliated with BDB LLC, our products, or our websites. They do not implicitly sponsor or endorse BDB LLC or any of our products or services.